Aiming at the problems of current aviation card readers, include poor portability, slow speed and tags' little capacity, a design method of large capacity Radio Frequency Identification (RFID) system based on STM32 was proposed. Using STM32 microprocessor as a core and adopting CR95HF radio chip, a new handled RFID card reader which worked in High Frequency (HF) and supported ISO 15693, ISO 18092 protocols was designed. The design of power, antenna and optimization of software speed, error rate was discussed in detail. A new large compiled capacity passive tag was also designed whose capacity is up to 32KB to form a large capacity RFID system with card reader. The experimental results show that, compared with the traditional card reader, the reading and writing speed of the card reader increases by 2.2 times, error rate reduces by 91.7% and tag capacity increases 255 times. It provides a better choice for fast, accurate and high data requirements of aviation logistics.